Press Releases

Creative Biogene, one of the top biotechnology products and services providers, is dedicated to offering a wide range of products and services that span the entire drug discovery and development continuum and can be tailored to specific research conditions. Creative Biogene provides customized molecular biology reagents and professional technical services to customers in the drug discovery, biomedical and life science research fields. Recently, Creative Biogene announced the release of its adenovirus construction and virus packaging in order to support related research.

Creative Biogene is a leading specialist in the manufacture of adenoviruses for gene delivery and gene therapy applications. From small adenovirus packaging for in vitro work to large-scale adenovirus packaging for animal research to adenovirus cGMP production for clinical applications. Regardless of the stage of a client's research, a team of scientists will collaborate to provide a custom adenovirus service that best meets the client's needs.

Creative Biogene uses a validated assay to measure the physical titer (virion) of all adenovirus packaging services using qPCR and primers for adenovirus-specific genes and by comparison to a standard curve of plasmid samples of known concentrations to quantify. 

Two types of vectors are required for recombinant adenovirus packaging: a shuttle vector (GOI) containing the gene of interest and a recombinant adenovirus vector (pAD). To ensure safe use, we use non-replicating (E1/E3 gene deletion) human adenovirus type 5 (Ad5). The first step in adenovirus production involves the transfer of the GOI from the shuttle vector to pAD by recombination of the left and right homology arms.

For large-scale and preclinical adenovirus packaging services, Creative Biogene's viral particles are then purified using IDX gradient ultracentrifugation to remove impurities and ensure safe use in vivo. Virus titers were then determined using qPCR. Adenoviral particles are then shipped overnight and can be used immediately for in vitro or in vivo studies.

“In addition to our adenovirus production services, we also offer catalog viral vector products including control viruses, ORFs, miRNAs, shRNAs, and more.” said Marcia Brady, the marketing director of Creative Biogene, she also claimed, “We have developed specialized platforms to assist in all areas of viral vector design and construction, as well as rapid turnaround virus production.”

About Creative Biogene

Creative Biogene, as a top-of-its-kind company that holds a leading position and is committed to accelerating the development of human science through biotechnology, offering innovative technologies, products, unique tools and services for research discoveries and product development. With more than 10 years of experience and in-house experts, Creative Biogene has become a well-recognized industry leader to support researchers worldwide.