CRISPR/cas9 platform, the division of Creative Biogene, is run by an international team of scientists with academic and industrial backgrounds, providing comprehensive gene editing services and products by using CRISPR/Cas9 technology. With the excellent CRISPR/Cas9 platform and experienced scientists, Creative Biogene is dedicated to providing reliable and professional services and products for clients at affordable prices. Recently, Creative Biogene announced the release of its knockout mouse models to help understand the biological mechanisms of genes and diseases in humans.

Creative Biogene provides high-quality, validated knockout mouse models for generating consistent research data, enabling research reproducibility and reducing overall research costs. The end-to-end commitment ensures adherence to project timelines, confidentiality, the exclusivity of intellectual property, breeding to SOPF standards, and global delivery.

Constitutive knockout mice are engineered to carry a gene that doesn't work. Typically, the inactivation of a gene is achieved by deleting (part or all) of its sequence, and it is nonfunctional in the whole animal. Conditional knockout mice provided by Creative Biogene allow gene deletion in a tissue- and/or time-specific manner. Typically, conditional KO mice are achieved through the Cre-lox system. Instead of deleting key sequences, it is flanked by loxP sites (called floxed sequences). Cre recombinase deletes the sequence between the two loxP sites. Inducible or tissue-specific Cre is used to knock out gene function in that tissue only.

Conditional knockout/inducible mice, inducible knockout mice, and knockdown mice from Creative Biogene avoid the risk of lethal phenotypes associated with gene inactivation at early stages of development and allows analysis of the effects of gene inactivation in adult animals. If an unexpected phenotype emerges in a knockout mouse population, Creative Biogene researchers using appropriate wild-type and/or HE controls will help assess where exactly the phenotypic changes in knockout mice come from. If the phenotype is induced by a mutated target gene, the control should not show that phenotype.

Creative Biogene creates new knockout mice through CRISPR/Cas9* genetic engineering, always backcrossing the first KO mouse female with a wild-type male to refresh/swap the Y chromosome. Then in subsequent breedings, the transgenic males obtained from the first round of breeding are used to mate with wild-type females.

“We have created a collection of more than 20,000 genetically engineered mouse models for research and drug development.” said Marcia Brady, the marketing director of Creative Biogene, she also added, “Our team of experts also provides a wide range of preclinical services including mouse model customization, pharmacology services and CRISPR/Cas9 gene editing to satisfy specific needs.”

About Creative Biogene

Creative Biogene is a top-of-its-kind company that holds a leading position and is committed to accelerating the development of human science through biotechnology, offering innovative technologies, products, unique tools, and services for research discoveries and product development. With more than 10 years of experience and in-house experts, Creative Biogene has become a well-recognized industry leader to support researchers worldwide.